J621 OR J602 - Medical Microbiology
Course director. Lecture content includes basic and infectious
disease bacteriology (24 hours total), medical mycology (3 hours), medical
virology (14 hours) and human parasitology (9 hours). The course laboratory
section includes applied immunology, bacterial culture and staining, and fungus
and parasite identification. The IUSM ANGEL course management system is used to
provide lecture image files and pdf files for all course topics. X510 - Concepts of Health and Disease
This course uses a problem-based learning format (PBL) to convey patient case information.
Students who successfully complete this course are prepared in basic clinical
skills such as venipuncture, throat swab, blood pressure and pulse
determination. Students learn the basics of laboratory methods such as bacterial
staining, hematocrit determination, differential blood cell counts, electrolyte
measurements, and interpretation of clinical laboratory results. The course
emphasizes the nine clincial competencies that are the basis for IU School of
Medicine's Competency-Based Curriculum. LIFS 692--Graduate Research in
Life Sciences 15 years (1991-Present): Research training for graduate
students. Methods include 2D gel electrophoreis, proteomics and
inflammation protein characterization. LIFS 492--Undergraduate Research in Life Sciences
and LIFS 192--Undergraduate Research in Life Sciences 13 years
(1993-Present): Research training for undergraduate students
DR. JOHNSON'S RESEARCH INTERESTS
Electromagnetic Field Exposure Influence on Biological Systems
Proteomics analysis of inflammation using acute tendonitis and chronic
rheumatoid arthritis models
Pulsed field therapy of inflammatory injury and modulation of in vivo
function: biochemical and biophysical measures
Dr. Johnson's primary research area focuses on the effects of
pulsed field therapy on biological processes
including immune system function. Pulsed fields of many types are increasingly recognized to modulate
inflammation and are widely investigated as a complementary therapy for
several disease conditions. Pulsed fields currently are used clinically for the
treatment of musculoskeletal disorders and are being evaluated as an
alternative to more invasive procedures such as electroconvulsive shock
therapy used for treatment of drug-refractory depression. We have developed rat models for the treatment of soft tissue injury and chronic joint disease.
We currently are evaluating
several pulsed field-generating medical devices with a goal to
establish a mechanism for the action of these fields at the cellular and tissue
levels. Experiments performed in conjunction with graduate and undergraduate
students consistently have shown field effects on both T-lymphocytes in
vitro and on the inflammation associated with injury and disease in
vivo. Techniques used in current investigations include lymphocyte
isolation, cell culture, blood chemistry, affinity chromatography, SDS protein gel
electrophoresis, 2D gel analysis, immunoblot analysis, ICAT proteomics,
immuohistochemistry, receptor
autoradiography, and in vivo functional analyses.
Bacteriology Research
Community-based Staphylococcus isolates and methicillin resistance
Molecular epidemiology
Microbial sampling in community environments
Research in Medical Education
Webpage dissemination for medical microbiology
Case development for problem-based learning
Script Concordance Testing for Problem Solving Skill
Electronic portfolios for documenting student accomplishment in the Competency-Defined Curriculum
Competency Coordinator for IU School of Medicine - Terre Haute
Statewide Competency Director for Problem Solving, IU School of Medicine
Selected Peer Reviewed Publications and Presentations
Anderson, M; Dannefer, E; Cottingham, A; Litzelman, D; Jackson, S; Johnson, MT; Cheney, D; Schmidt, B and Walling, AD (2007)
"Use of Medical Student Portfolios for Teaching and Assessing Core Competencies" Medical Education Resources Exchange Session presentation,
AAMC Central Group on Educational Affairs, Indianapolis, IN
Johnson, MT; (2007) "Impact of Online Learning Modules on Medical Student Microbiology Examination Scores"
poster, AAMC Central Group on Educational Affairs, Indianapolis, IN
Dillon, B; Johnson, MT; and Polin, M (2007) "Programs for Tracking Medical Learning: A Student Perspective"
poster, AAMC Central Group on Educational Affairs, Indianapolis, IN
Dortch, A. and Johnson, MT (2006) Characterization of Pulsed Magnetic Field Therapy in a
Rat Model for Rheumatoid Arthritis Biomedical Sciences Instrumentation, 42:
302-307.
Owegi, R. and Johnson, MT (2006) Localized Pulsed Magnetic Fields for Tendonitis Therapy. Biomedical Sciences
Instrumentation, 42: 428-433.
Johnson, MT and Nindl, G (2005) Clinical Science:
Laboratory and Problem Solving. ProQuest Information and Learning, Ann
Arbor, MI (medical textbook)
Ramirez-Icaza, G, Mohammed, KA, Nasreen, N, Van Horn, RD, Hardwick, JA,
Sanders, KL, Tian, J, Ramirez-Icaza, C, Johnson, MT, and V Antony (2003) Th2 Cytokines IL-4 and IL-13 Down
Regulate Paxillin Expression in Bronchial Airway Epithelial Cells, Journal
of Clinical Immunology 24: 426-34.
Johnson, MT, Waite, LR; and Nindl, G (2004) Noninvasive Treatment
of Inflammation Using Electromagnetic Fields: Current and Emerging
Therapeutic Potential. Biomedical Sciences Instrumentation, 40:
469-474.
Nindl, G; Balcavage, WX; Moulton, C; Waite, LR; and Johnson, MT (2004)
Effect of Hydrogen Peroxide on Jurkat T Cells and Its Regulation by Low
Frequency EMFs. Biomedical Sciences Instrumentation, 40: 123-128
Nindl, G, Johnson, MT and Balcavage, WX (2003) Low-frequency
electromagnetic field effects on lymphocytes: Potential for treatment of
inflammatory diseases. In: Clinical Applications of Bioelectromagnetic
Medicine. Paul Rosch and Marko Markov (Eds.). Marcel Dekker Inc., New
York, NY, U.S.A.
Johnson, MT, McCullough, J, Nindl, G, and Chamberlain, JK (2003)
Autoradiographic Evaluation of Electomagnetic Field Effects on Serotonin
(5-HT1A) Receptors in Rat Brain Biomedical Sciences
Instrumentation, 39
Harris, RD, Nindl, G, Balcavage, WX, Weiner, W, McLean, H, and
Johnson, MT (2003) Use Of Proteomics Methodology To Evaluate
Inflammatory Protein Expression In Tendinitis. Biomedical Sciences
Instrumentation, 39
Sharma, HA, Balcavage, WX, Waite, LR, Johnson, MT, and Nindl, G
(2003) Characterization of a Real time H2O2 Monitor for use in studies on
H2O2 production by antibodies and cells. Biomedical Sciences
Instrumentation, 39
Wetzel, B, Nindl, G, Vesper, D, Swez, JA, and Johnson, MT
(2002) Quantitative Characterization of Rat Tendinitis to Evaluate the
Efficacy of Therapeutic Interventions. Biomedical Sciences
Instrumentation, 38: 157-162.
Rabenberg, VS, Ingersoll, CD, Sandrey, MA and Johnson, MT
(2002) The bactericidal and cytotoxic effects of antimicrobial wound
cleansers. J. Athletic Training, 37 (1), 51-54.
Johnson, MT, Vanscoy-Cornett, A, Chamberlain, JC, Seaward, MB, and
Nindl, G (2001) Electromagnetic Fields Used Clinically To Improve
Bone Healing Also Impact Lymphocyte Proliferation In Vitro. Biomedical
Sciences Instrumentation 37: 215-220.
Sandrey, MA, Vesper, DN, Johnson, MT, Nindl, G, Swez, JA, Chamberlain,
J and Balcavage, WX (2001) Effect of electromagnetic
fields on rat mass in short-duration studies. Bioelectromagnetics,
23: 2-6.
johnsomt (at) iupui.edu
